R E C I P E S

II. Polymerase Chain Reaction

1% Cresol Red Dye

Makes 50 ml. Store at room temperature.

  1. Add 500 mg to 50 ml of distilled water in a 50-ml tube.
  2. Shake tube to dissolve.

Cresol Red Loading Dye

Makes 50 ml. Store at 4░ C.

  1. Add 17 g of sucrose to 49 ml of distilled water in a 50-ml tube.
  2. Shake tube to dissolve
  3. Add 1 ml of 1% cresol red dye.
  4. Shake tube to mix.

Mt Control Region Primer/Loading Dye Mix

Makes for 50 PCR reactions. Store in freezer for 1 year.

645 Ál of distilled water
460 Ál of Cresol Red Loading Dye
10 Ál of 5' primer [15 pm/Ál]
10 Ál of 3' primer [15 pm/Ál]
Vortex to mix.

Mt Control Region Primer Sequences

5'-TTAACTCCACCATTAGCACC-3'

5'-GAGGATGGTGGTCAAGGGAC-3'



M I T O C H O N D R I A L  C O N T R O L  R E G I O N

DNA Learning Center, Cold Spring Harbor Laboratory
Noncommercial, educational use only.
Kits available from Carolina Biological Supply Company.